Isolation of Dna from Hog or Calf Thymus

Published: 2021-06-29 06:44:36
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Category: Science

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Experiment No. 5
ISOLATION OF DNA FROM HOG OR CALF THYMUS

Methodology
Materials:
0.15 M NaCl-0.014 M sodium citrate buffer pH 7.4
2.0 M NaCl 95% ethanol
70% ethanol 0.10 M acetate buffer pH 4.6
Diphenylamine reagent Hog or calf thymus
5% trichloroacetic acid (TCA)
0.1% DNA solution
Equipments:
Top loading balance Homogenizer or blender
Centrifuge Thermometer
Micropipettes and pipette tips
1 mL quartz cuvette Stirring rod
UV spectrophotometer

Procedure
A. Extraction of DNA
1. Remove residual fat, blood vessels, and other extraneous tissues from hog or calf thymus.
2. Weigh 12 g of thymus and mince finely.
3. Add 0.15 M NaCl-0.01 M sodium citrate buffer, pH 7.4 to a final volume of 30 mL. Homogenize

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